Open Access Open Access  Restricted Access Subscription or Fee Access

Blend of biocompatible Magnesium oxide nano molecule and its anticancer action against human colon cell line

Jeba Jane Ratney

Abstract


Metal oxides occupy a critical part in numerous zones of science, and material science. Metal oxide nano materials are vital and fantastic materials. Present work concentrated on to union of MgO nanoparticle by Green technique utilizing [6]-Gingerol, were subjected to sub-atomic docking ponders for the restraint of leukotriene A4 hydrolase (LTA4H) protein is viewed as a significant focus for disease treatment. In silico expectation utilizing a docking approach uncovered that LTA4H may be a potential focus of MgO np utilizing [6]-gingerol. It stifles safe haven autonomous tumor cell development by restraining LTA4H action in HT 29 colorectal disease cells. All things considered, these discoveries show a critical part of LTA4H in malignancy furthermore bolster the anticancer adequacy of MgO np utilizing [6]-gingerol focusing of LTA4H for the counteractive action of colorectal disease. The actuation space of LTA4H docked with the MgO np utilizing [6]-gingerol demonstrated the docking scoring of - 218.92, Kcal.mol-1. The outcomes uncovered that MgO np utilizing [6]-gingerol is considered as a decent inhibitor of LTA4H. The incorporated Magnesium oxide nanoparticles have been described by auxiliary, optical, warm, and indicated powerful cytotoxic impact against HT29 colon growth cell line with in IC50 estimation of 25 µg/ml by the MTT assay. The present study shows the green incorporated Magnesium oxide nanoparticle is utilized noteworthy higher anticancer movement. From the outcomes got it is recommended that the green combined MgO nanoparticle which is a compelling anticancer specialist. Keywords: Cytotoxic Effect, [6]-gingerol, LTA4H, Metal oxides, Nontoxic nature

Full Text:

PDF

References


Veeradate Piriyaawong, Voranuch Thongpool, Piyapong Asanithi and Pichet Limsuwan,”Preparation and characterization of Alumina Nanoparticles in Deionized Water Using Lazer Abalation Technique,” Hindawi Publishing Cooperation Journal of Nanomaterials, 819403, pp.1-6,2012.

Sawai, J., Kojima, H., Igarashi, H., Hashimoto, A., Shoji, S., Sawaki, T., Hakoda, A., Kawada, E., Kokulan, T. And Shimizu,M., Antibacterial characteristics of magnesium oxide powder. World J. Microbial. Biotechnol., 16,187-194 (2000)

Shi, L.E., Fang, X.J.,Zhang, Z. L., Zhou, T., Jiang, D., Wu, H.H. and Tang,Z.X., Preparation of nano-ZnO using sonication method and its antibacterial characteristics. Int.J.Food sci. Tech., 47,1866-1871 (2012)

Tony Jin and yipping He, “Antibacterial Activities Of Magnesium Oxide (MgO) Nanoparticle Against Foodborne Pathoogens,” J Nanopart Res, 13, pp. 6877-6885, 2011.

K.Mageshwari, Sawanta S. Mali,R. Sathyamoorthy and Pramod S. Patil, “Template-free synthesis of MgO nanoparticles for effective photocatalytic applications,” Powder Technology, 249,pp.456-462, 2013.

M H. Bar, D. K. Bhui, G. P. Sahoo, P. Sarkar, S. Pyne, A. Misra, “Green synthesis of silver nanoparticles using seed extract of Jatropha curcas.” Colloid Surf. A-Physicochem. Eng. Asp., vol 348, 2009, pp.212-216.

K. Prasad, A. K. Jha, “ZnO nanoparticles: synthesis and adsorption study.” Natural Science, vol 1, 2009, pp. 129-135.

R. Wahab, S.G. Ansari, M.A. Dar, Y.S. Kim, H.S. Shin, Materials Science Forum 2007, 558-559, 983.

Banerjee S, Mullick HI and Banerjee J, Zingiber officinale: A Natural Gold. International Journal of Pharma and Biosciences, 2, 2011, 283-294.

Behera S, Ojha AK, Rout J and Nayak PL, Plant mediated synthesis of silver nano particles: opportunity and challenges. International Journal of Biology Pharmacy and Allied science, 1, 2012, 1637-1658.

Hosseinkhani P, Zand AM, Imani S, Rezayi M, Zarchi RS, Determining the antibacterial effect of ZnO nanoparticle against the pathogenic bacterium Shigella dysenteriae (type 1). International Journal of Nano Dimension, 4, 2011, 279-285.

Ipsa S and Nayak PL, Antimicrobial Activity of Copper Nanoparticles Synthesised by Ginger (Zingiber officinale) Extract. World Journal of Nano science & Technology, 1, 2013, 10-13.

Cross AJ, Sinha R. Meat-related mutagens/carcinogens in the etiology of colorectal cancer. Environmental and Molecular Mutagenesis 2004; 44(1):44–55.

Jägerstad M, Skog K. Genotoxicity of heat-processed foods. Mutation Research 2005; 574(1–2):156–172.

Kiuchi, F., Iwakami, S, Shibuya, M., Hanaoka, F and Sankawa, U. (1992) Inhibition of prostaglandin and leukotriene biosynthesis by gingerols and diarylheptanoids. Chemical and Pharmaceutical bulletin, 40, 387-391.

Samuelsson B, Dahlen SE, Lindgren JA, Rouzer CA, Serhan CN. Leukotrienes and lipoxins: structures, biosynthesis, and biological effects. Science 1987;237: 1171–6.

Funk CD. Prostaglandins and leukotrienes: advances in eicosanoid biology. Science 2001;294:1871–5.

Fabre JE, Goulet JL, Riche E, et al. Transcellular biosynthesis contributes to the production of leuko- trienes during inflammatory responses in vivo. J Clin Invest 2002;109:1373–80.

Chen X, Wang S, Wu N, Yang CS. Leukotriene A4 hydrolase as a target for cancer prevention and therapy. Curr Cancer Drug Targets 2004;4:267–83.

Orning L, Krivi G, Fitzpatrick FA. Leukotriene A4 hydrolase. Inhibition by bestatin and intrinsic amino- peptidase activity establish its functional resemblance to metallohydrolase enzymes. J Biol Chem 1991;266: 1375–8.


Refbacks

  • There are currently no refbacks.