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Trichoderma Based Synthesis of Silver Oxide Nanoparticles, Their Characterization and Assessment of Antifungal Activity

Shazia Parveen, Abdul Hamid Wani, Henam Sylvia Devi, Mohammad Ashraf Shah, Mohd Yaqub Bhat

Abstract


The fungal spore suspension of Trichoderma harzianum was used for the preparation of AgO nanoparticles. T. harzianum spore suspension acts as reducing and stabilizing agent for the fabrication of nanoparticles. The synthesized nanoparticles were characterized by X-ray diffractometry (XRD), scanning electron microscopy (SEM), energy dispersive X-analysis (EDAX) and transmission electron microscopy (TEM). The AgO nanoparticles were circular in shape with average diameter 10-20 nm. The AgO nanoparticles showed promising antifungal activities against all the tested fruit rot fungal pathogens like, Penicillium chrysogenum, Trichothecium roseum and  Aspergillus niger. However, highest effective against A. niger and P. chrysogenum as maximum zone of inhibition and lowest minimum inhibitory concentration value (0.032) of AgO nanoparticles was found against these two fungal pathogens. Activity index of these synthesized AgO NPs were also found highest for A. niger followed by P. chrysogenum and least for T. roseum.


Keywords


Nanoparticles, Trichoderma harzianum, antifungal activity, ecofriendly

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References


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