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New nanomagnetic bead dot ELISA immunoassay and its application to the detection of Giardial amblia copro antigen

Navni Bajpai, Sanjeev Singh

Abstract


The discovery of vegetative forms or cysts of Giardia lamblia in the faeces of infected individuals is the conventional foundation of diagnosis. It has recently been used rather than techniques that rely on subjective microscopy of stool specimens of Giardia cysts, which can facilitate the diagnosis of Giardia lamblia in faecal specimens, with the advent of more objective techniques such as antigen detection, which can facilitate the diagnosis of Giardia lamblia in faecal specimens. Dot ELISA detects amoeba, malaria, toxoplasmosis, babesiosis, cutaneous and visceral leishmaniasis, tripanosomasis, fasciolosis, toxoplasmosis, fasciolosis, cystosis, echinococcosis, trixinosis, and even tickling. This test is commonly used to diagnose leishmaniasis. Metal nanoparticles are becoming more popular in biomedical research. The purpose of this study was to develop a novel nano diagnostic test (ELISA based on nanomagnetic beads and dot ELISA assay utilising nanomagnetic beads) for detecting coproantigen in Giardia lamblia patients' stool. It was a metaphor for a classic sandwich. ELISA. This is the first study to use NMB dot ELISA to detect G. Giardia lamblia antigen in the faeces of infected patients.


Keywords


Giardia lamblia, Dot ELISA, Nano technology, Nanomagnetic, Antigen.

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References


Feng, Y. and Xiao, L., 2011. Zoonotic potential and molecular epidemiology of Giardia species and giardiasis. Clinical microbiology reviews, 24(1), pp.110-140.]

Barry, M.A., Weatherhead, J.E., Hotez, P.J. and Woc-Colburn, L., 2013. Childhood parasitic infections endemic to the United States. Pediatric Clinics, 60(2), pp.471-485.

Coelho, C.H., Costa, A.O., Silva, A.C.C., Pucci, M.M., Serufo, A.V., Busatti, H.G.N.O., Durigan, M., Perales, J., Chapeaurouge, A., da Silva e Silva, D.A. and Gomes, M.A., 2016. Genotyping and descriptive proteomics of a potential zoonotic canine strain of Giardia

duodenalis, infective to mice. PLoS One, 11(10), p.e0164946.

Núñez, F.A., Hernández, M., and Finlay, C.M., 1999. Longitudinal study of giardiasis in three-day care centres of Havana City. Acta tropica, 73(3), pp.237-242.

Pappas, M.G., 1988. Recent applications of the Dot-ELISA in

immunoparasitology. Veterinary Parasitology, 29(2-3), pp.105-129.

Venkataramana, M., Rashmi, R., Uppalapati, S.R., Chandranayaka, S., Balakrishna, K.,Radhika, M., Gupta, V.K. and Batra, H.V., 2015. Development of sandwich dot-ELISA for specific detection of Ochratoxin A and its application on to contaminated cereal grains originating from India. Frontiers in microbiology, 6, p.511.

Arjmand, J., Esmaeilnejad, B., Jalali, M.H.R., Ghorbanpoor, M. and Froushani, S.M.A.,2014. Designing and evaluation of Dot-ELISA for diagnosis of Fasciola infection in cattle.In Veterinary research forum: an international quarterly journal (Vol. 5, No. 2, p. 141).Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.

Moharm, I.M., El Enain, G., Bahaa El Deen, W., Mahgoub, A., Hendawy, M. and Aly, I.,2014. Evaluation of nano-gold beads based-ELISA for detection of Giardia lamblia antigen in stool samples of infected patients. MEJSR, 21(12), pp.2264-73.

Tiwari, P.M., Vig, K., Dennis, V.A. and Singh, S.R., 2011. Functionalized gold nanoparticles and their biomedical applications. Nanomaterials, 1(1), pp.31-63.

El-Ansary, A. and Al-Daihan, S., 2009. On the toxicity of therapeutically used nanoparticles: an overview. Journal of Toxicology, 2009.

Blagg, W., Schloegel, E.L., Mansour, N.S. and Khalaf, G.I., 1955. A new concentration technic for the demonstration of protozoa and helminth eggs in feces. American Journal of Tropical Medicine and Hygiene, 4(1), pp.23-8.

Mezo, M., González-Warleta, M., Carro, C. and Ubeira, F.M., 2004. An ultrasensitive capture ELISA for detection of Fasciola hepatica coproantigens in sheep and cattle using a new monoclonal antibody (MM3). Journal of Parasitology, 90(4), pp.845-852.

Guobadia, E.E. and Fabemi, B.O., 1997. The isolation of Fasciola gigantica-specific antigens and their use in the serodiagnosis of fasciolosis in sheep by the detection of circulating antigens. Veterinary parasitology, 68(3), pp.269-282.

Fagbemi, B.O., Obarisiagbon, I.O. and Mbuh, J.V., 1995. Detection of circulating antigen in sera of Fasciola gigantica-infected cattle with antibodies reactive with a Fasciola-specific 88-kDa antigen. Veterinary parasitology, 58(3), pp.235-246.

Tijssen, P. and Kurstak, E., 1984. Highly efficient and simple methods for the preparation of peroxidase and active peroxidase-antibody conjugates for enzyme immunoassays. Analytical biochemistry, 136(2), pp.451-457.


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