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Evaluation of Antimicrobial Activity using Zinc Oxide Nanoparticles

Navni Bajpai, Sanjeev Singh

Abstract


The goal of the study was to use common laboratory techniques to create Zinc Oxide nanoparticles in a short amount of time and to check the antibacterial activity of zinc oxide nanoparticles on gram negative and gram positive bacteria. Wet chemical technique was used to make zinc oxide nanoparticles. Activity augmentation for Escherichia coli and Staphylococcus aureus were studied when used in combination with Zinc Oxide Nanoparticles. The Nps are characterized by UV visible spectroscopy. The particle size and form of nanoparticles alter when different salt precursors and calcination temperatures are used. The form of ZnO NPs is greatly impacted by their optical property, as seen by the variance in UV-Vis absorption peaks. The successful synthesis of ZnO NPs is confirmed by the vibrational phonon of ZnO measured in the range of 433 cm-1-510 cm-1. Disc diffusion method is used to check the minimum inhibitory concentration (MIC). Zone of inhibition measured through vernier caliper


Keywords


ZnO Nanoparticles, Escherichia coli and Staphylococcus aureus, Zinc Nitrate, minimum inhibitory concentration

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