Open Access Open Access  Restricted Access Subscription or Fee Access

Green Synthesis of Silver Nanoparticles Using Leaf Extract of Calpurnia Aurea and Evaluation of its Antibacterial Activity

Birkinesh Girma, Gezahegn Faye, Tilahun Wubalem

Abstract


Synthesis of nanoparticles using plant extracts is an emerging as a new and effective method that avoids the involvement of toxic chemicals. In this study AgNPs synthesized using Calpurnia aurea leaf extracts were evaluated for their antibacterial activity. The green synthesized AgNPs were characterized using analytical instruments like UV–visible, X-ray diffraction (XRD) and Fourier transform infrared (FTIR). The UV-Vis spectra shows surface Plasmon resonance peak present at 429 nm. The observed FTIR spectra of the extract exhibited some degree shift in the corresponding nanoparticles, this result clearly showed that the extracts contain functional groups act in capping the nanoparticles. XRD analysis revealed that silver nanoparticle was crystalline in nature and has face-centered cubic geometry. The antibacterial activity of both AgNPs and extract were evaluated against Bacillus subtilis and Escherichia coli. Bacteria using agar well diffusion assay. The synthesized AgNPs showed enhanced antibacterial potential against Bacillus subtilis than Escherichia coli.


Keywords


Antibacterial, Silver nanoparticles, Bacillus subtilis, Escherichia coli, Calpurnia aurea

Full Text:

PDF

References


El-Shahaby, O., El-Zayat, M., Salih, E., El-Sherbiny, I. M. and Reicha, F.M. Evaluation of antimicrobial activity of water infusion plant-mediated silver nanoparticles. J. Nanomed. Nanotechol. 2013, 4, 178.

G. Morose, The 5 principles of “Design for Safer Nanotechnology,” J. Clean. Prod. 18 (2010) 285–289. https://doi.org/10.1016/j.jclepro.2009.10.001.

Allafchian, A. R., Mirahmadi-Zare, S.Z., Jalali, S.A.H., Hashemi, S.S. and Vahabi, M.R. Green synthesis of silver nanoparticles using phlomis. J. Nanostructure in Chem. 2016, 6,129–135.

Sondi, I. and Salopek-Sondi, B. Silver nanoparticles as antimicrobial agent: a case study on E.coli as a model for Gram-negative bacteria. Journal of colloid and interface science. 2004, 275,

–182.

Ananthi, P.; Jeyapaul, U.; James, A.; Balgan, A.S.; Mary J.K. Green synthesis and characterization of silver nanoparticles using TriumfettaRotundifolia plant extract and its antibacterial activities. J. Nat. Prod. Plant Resour. 2016, 6, 21-27.

Baker, C., Pradhan, A., Pakstis, L., Pochan, D.J. and Shah, S.I. Synthesis and antibacterial properties of silver nanoparticles. J. nanosci. and nanotechnol., 2005, 5, 244–249.

Latha, N. and Gowri, M. Biosynthesis and characterisation of Fe3O4 nanoparticles using Caricaya papaya leaves extract. Int J Sci Res, 2014, 3, 1551–1556.

Li, X.Q., Elliott, D.W. and Zhang, W.X. Zero-valent iron nanoparticles for abatement of environmental pollutants: materials and engineering aspects. Critical reviews in solid state and materials sciences, 2006, 31, 111–122.

Makarov, V.V., Love, A.J., Sinitsyna, O.V., Makarova, S.S., Yaminsky, I.V., Taliansky, M.E. and Kalinina, N.O. “Gre nanoparticles using plants. Acta Naturae. 2014, 6, 20.

Ren, Y.Y., Yang, H., Wang, T. and Wang, C. Green synthesis and antimicrobial activity of monodisperse silver nanoparticles synthesized using Ginkgo Biloba leaf extract. Physics Letters A, 2016, 380, 3773–3777.

Zhu, Z., Piao, S., Myneni, R.B., Huang, M., Zeng, Z., Canadell, J.G., Ciais, P., Sitch, S.,Friedlingstein, P., Arneth, A. and Cao, C. Greening of the Earth and its drivers. Nature climate change, 2016, 6, 791–795. Thenmozhi, B.; suryakiran, S.; sudha, R.; revathy, B. Green synthesis and comparative study of silver and iron nanoparticle from leaf extract. int. J. instit. pharm and life sci. 2014, 4, 1–19.

Pattanayak, M. and Nayak, P.L. Green synthesis and characterization of zero valent iron nanoparticles from the leaf extract of Azadirachta indica (Neem). World J. Nano Sci. Technol, 2013, 2, 06–09.

Hebbalalu, D., Lalley, J., Nadagouda, M.N. and Varma, R.S. Greener techniques for the synthesis of silver nanoparticles using plant extracts, enzymes, bacteria, biodegradable polymers, and

microwaves. ACS Sustainable Chemistry & Engineering, 2013, 1,703–712.

Njagi, E.C., Huang, H., Stafford, L., Genuino, H., Galindo, H.M., Collins, J.B., Hoag, G. E. and Suib, S.L. Biosynthesis of iron and silver nanoparticles at room temperature using aqueous sorghum bran extracts. Langmuir. 2010, 27, 264–271.

Amit, K Mittal.; Yusuf, Chisti.; Uttam, Ch. B. Synthesis of metallic nanoparticles using plant extracts. J. Biotechnol. Adv. 2013, 31, 346–356

Vijayaraghavan, K., Nalini, S.K., Prakash, N.U. and Madhankumar, D. Biomimetic synthesis of silver nanoparticles by aqueous extract of Syzygium aromaticum. Materials Letters, 2012, 75, 33–35.

Jagtap, U.B. and Bapat, V.A. Green synthesis of silver nanoparticles using Artocarpus heterophyllus Lam. seed extract and its antibacterial activity. Industrial Crops and Products, 2013,46,132–137.

Edison, T.N.J.I., Baral, E.R., Lee, Y.R. and Kim, S.H. Biogenic synthesis of silver nanoparticles using Cnidium officinale extract and their catalytic reduction of 4-Nitroaniline. Journal of Cluster Science, 2016. 27, 285–298.

Ndikau, M., Noah, N.M., Andala, D.M. and Masika, E. Green Synthesis and Characterization of Silver Nanoparticles Using Citrullus lanatus Fruit Rind Extract. International journal of analytical chemistry, 2017, 2017.

Wang, T., Lin, J., Chen, Z., Megharaj, M. and Naidu, R. Green synthesized iron nanoparticles by green tea and eucalyptus leaves extracts used for removal of nitrate in aqueous solution. Journal

of cleaner production, 2014, 83, 413–419.

Ghaedi, M., Yousefinejad, M., Safarpoor, M., Khafri, H.Z. and Purkait, M.K. Rosmarinus officinalis leaf extract mediated green synthesis of silver nanoparticles and investigation of its antimicrobial properties. Journal of Industrial and Engineering Chemistry, 2015, 31, 167–172.

Zohra, S.F., Meriem, B., Samira, S. and Alsayadi-Muneer, M.S. Phytochemical screening and identification of some compounds from mallow. J Nat Prod Plant Resour. 2012, 2, 512–516.

Yang, N. and Li, W.H. Mango peel extract mediated novel route for synthesis of silver nanoparticles and antibacterial application of silver nanoparticles loaded onto non-woven fabrics. Industrial Crops and Products, 2013, 48, 81–88.

Pethakamsetty, L., Kothapenta, K., Nammi, H.R., Ruddaraju, L.K., Kollu, P., Yoon, S.G. and Pammi, S.V.N. Green synthesis, characterization and antimicrobial activity of silver nanoparticles using methanolic root extracts of Diospyros sylvatica. J.Envi. Sci., 2017, 55, 157–163.

Fadel, Q.J. and Al-Mashhedy, L.A.M. Biosynthesis of Silver Nanoparticles Using Peel Extract of Raphanus sativus L. BioTechnol: An Indian Journal, 2017, 13.

Ajayi, E. and Afolayan, A. Green synthesis, characterization and biological activities of silver nanoparticles from alkalinized Cymbopogon citratus Stapf. Advances in Natural Sciences: Nanosci. and Nanotechnol. 2017, 8, 15–17.

Sarkar, D.; Paul, G. Green synthesis of silver nanoparticles using Mentha asiatica (Mint) extract and evaluation of their antimicrobial potential, Int. J. Curr. Res. Biosci. Plant Biol. 2017, 4, 77– 82.

Mohanta, Y.K., Panda, S.K., Bastia, A.K. and Mohanta, T.K. Biosynthesis of silver nanoparticles from Protium serratum and investigation of their potential impacts on food safety and control.

Frontiers in microbiology, 2017, 8, 626.

Ahmed, M.J., Murtaza, G., Mehmood, A. and Bhatti, T.M. Green synthesis of silver nanoparticles using leaves extract of Skimmia laureola: characterization and antibacterial activity. Materials Letters, 2015, 153, 10–13.

Bagherzade, G., Tavakoli, M.M. and Namaei, M.H. Green synthesis of silver nanoparticles using aqueous extract of saffron (Crocus sativus L.) wastages and its antibacterial activity against six bacteria. Asian Pacific Journal of Tropical Biomedicine, 2017, 7, 227–233.

Viswadevarayalu, A. Green Synthesis of Ag Nanoparticles by Helicteris Isora.L Fruit Extract as a Reducing Agent: Antimicrobial Activity. Inter. J. Adv. Res. Physic. Sci. 2015, 2, 34–42.

Saranyaadevi, K., Subha, V., Ravindran, R.E. and Renganathan, S.A.H.A.D.E.V.A.N. Green synthesis and characterization of silver nanoparticle using leaf extract of Capparis zeylanica. Asian J. Pharm. Clin. Res, 2014, 7, 44–48.

Bose, D. and Chatterjee, S. Antibacterial activity of green synthesized silver nanoparticles using Vasaka (Justicia adhatoda L.) leaf extract. Indian journal of microbiology, 2015, 55, 163–167.

Venkatesham, M., Ayodhya, D., Madhusudhan, A., Kumari, A.S., Veerabhadram, G. and Mangatayaru, K.G. A novel green synthesis of silver nanoparticles using gum karaya:Green Synthesis of Silver Nanoparticles Using Leaf Extract of Calpurnia Aurea Girma et al.characterization, antimicrobial and catalytic activity studies. Journal of Cluster Science, 2014, 25,409–422.

Ahmad, N. and Sharma, S. Green synthesis of silver nanoparticles using extracts of Ananas comosus. Green and Sustainable Chemistry, 2012, 2, 141.

Patra, J.K., Das, G. and Baek, K.H. Phyto-mediated biosynthesis of silver nanoparticles using the rind extract of watermelon (Citrullus lanatus) under photo-catalyzed condition and investigation of its antibacterial, anticandidal and antioxidant efficacy. J. Photochemistry and Photobiol. B: Biology, 2016, 161, 200–210.

Sudhakar, C., Selvam, K., Govarthanan, M., Senthilkumar, B., Sengottaiyan, A., Stalin, M. and Selvankumar, T. Acorus calamus rhizome extract mediated biosynthesis of silver nanoparticles and their bactericidal activity against human pathogens. Journal of Genetic Engineering and Biotechnology, 2015, 13, 93–99.

Zhiqiang, W.; Chunhe, Y.; Cheng, F.; Megharaj, M. Dye removal using iron–polyphenol complex nanoparticles synthesized by plant leaves. Env. Technol. Innov. 2014, 1–2 29–34.

Mittal, A.K., Chisti, Y. and Banerjee, U.C. Synthesis of metallic nanoparticles using plant extracts. Biotechnology advances, 2013, 31, 346–356.

Mengyu, G.; Xiulan, W.; Ting. W.; Zuliang, C. Biosynthesized iron-based nanoparticles used as a heterogeneous catalyst for the removal of 2, 4-dichlorophenol. Separation and Purification Technology. 2017, 175, 222–228.

Wang, L., Xie, J., Huang, T., Ma, Y. and Wu, Z. Characterization of silver nanoparticles biosynthesized using crude polysaccharides of Psidium guajava L. leaf and their bioactivities. Materials Letters. 2017, 208, 126–129.


Refbacks

  • There are currently no refbacks.